As shown in Figure 1A, Western blot analysis revealed that AEG-1 protein was highly expressed in NCI-H226 cells (from lung squamous cell carcinoma with high metastatic ability), whereas Slu-01 cells (from lung adenocarcinoma with low metastatic ability) had undetectable AEG-1 protein expression. This evidence concerns the gene MTDH and squamous cell lung carcinoma.