Again to confirm that our results were not cell line specific, we also compared total PAX3 mRNA expression levels in the three melanocyte cultures (neonatal NHEM-n, and adult NHEM-a (P) and NHEM-a (I)) and the eleven melanoma cell lines (primary MM200, MM229, MM329, MM540, MM622, WM115, and metastatic A2058, M14, SKMEL2, SKMEL5, UACC62 cell lines) (Fig 3B). Here, PAX3 is linked to melanoma.