We next measured PAX3 protein levels in three normal melanocyte cultures (neonatal NHEM-n, and adult NHEM-a (P) and NHEM-a (I)), and eleven melanoma cell lines (primary MM200, MM229, MM329, MM540, MM622, WM115, and metastatic A2058, M14, SKMEL2, SKMEL5, UACC62 cells) by both immunocytochemistry and western blot to ensure our observed results were not cell line specific. The gene discussed is PAX3; the disease is melanoma.