As an indirect immunofluorescense technique for IgM detection, Pneumoslide IgM can simultaneously diagnose 9 pathogens of infectious disease of the respiratory tract, including the Legionella pneumophila serogroup 1 (LP1), Mycoplasma pneumoniae (MP), Coxiella burnetii (COX), Chlamydophila pneumoniae (CP), Adenovirus (ADV), Respiratory syncytial virus (RSV), Influenza A (INFA), Influenza B (INFB), Parainfluenza 1, 2 and 3 (PIVs). This evidence concerns the gene CD40LG and infectious disease.