With bTB vaccine efficacy studies, long-term cultured (i.e., 14 days) IFN-γ ELISPOT responses to vaccination (i.e., BCG, M. bovis ΔRD1, and viral-vectored Ag85) negatively correlates with mycobacterial burden and TB-associated pathology and positively correlates with vaccine-induced protection [23–25]. This evidence concerns the gene IFNG and tuberculosis.