In order to examine whether Nanog interacts with p-Stat3 (Y705) directly in the nucleus of HCC cells, we analyzed the anti-Nanog-mediated immunoprecipitates from nuclear extracts by immunoblotting with anti-p-Stat3 (Y705), or anti-Nanog antibody, respectively, in 97 L cell lines. This evidence concerns the gene NANOG and hepatocellular carcinoma.