PROM1 and central nervous system cancer: Glioma stem like cell spheres of the 10th generation were assessed by immunofluorescence, 4.2 ± 1.29% SHG-139S cells in spheres were CD133+ (Figure 2, C1) and 84.12 ± 9.96% cells stained positive for A2B5 (Figure 2, C2).