EGF and oligodendroglioma: Secondary sphere formation assay confirmed that SHG-139S have self-renewal capacity; immunofluorescence confirmed the expression of Vimentin in cells with the neural stem cell marker Nestin+; when SHG-139S were induced to differentiate in NSCM containing serum and without bFGF and EGF, adherent cells were observed, and GFAP, the early neuronal marker β-III Tubulin and the oligodendroglioma marker GalC were expressed.