To explore the effects of a synthetic miR-27a mimic (mimic-27a), miR-27a AMO (AMO-27a), and a negative control mRNA (NC-27a) on TICAM-2 expression in the spinal cord after IR, we intrathecally injected mimic-27a, AMO-27a, and NC-27a for three days before ischemia, and then examined both the mRNA and protein expression of TICAM-2 by RT-PCR and western blotting, respectively. The gene discussed is TICAM2; the disease is ischemia.