In the present study, we describe for the first time the utilization of a state of the art-ELISA assay to obtain reliable data on the occurrence of IgG and IgM antibodies to GA1, GM1, GM2, GD1 a, GD1b, GQ1b gangliosides in a well characterized group of 84 ALS patients. This evidence concerns the gene CD40LG and amyotrophic lateral sclerosis.