To confirm the functional consequence of reduced HOXA5 expression within lung cancer cells, we evaluated the ability of stable HOXA5-overexpressing mixed cell clone (HOXA5 mix) and CL1-0 cells with highly expressed HOXA5 to invade through Matrigel-coated transwell filter membranes following transfection with HOXA5 siRNAs. Here, HOXA5 is linked to lung carcinoma.