RUVBL2 and hepatocellular carcinoma: These experiments were carried out in the human HCC cell line HuH7 transfected with an anti-Reptin siRNA, Etoposide treatment induced a 1.94 ± 0.13 fold increase in the phosphorylation of H2AX Reptin depletion by itself had no effect on H2AX phosphorylation.