When memory B cell subsets in these mice were assessed, we found as expected that the frequency of memory B cell population (i.e. CD19+GL7-Fas-CD80+PD-L2+) increased in the spleens of infected WT and Bam32-/- mice as the infection progressed (see S2 Fig for gating strategy). The gene discussed is PDCD1LG2; the disease is infection.