Throughout the infection, the levels of IFN-γ (Fig 2A), TNF-α (B) and IL-6 (C) in culture supernatant fluids of splenocytes from infected Bam32-/- mice were significantly (p < 0.05–0.01) higher than those from WT mice, with the difference being more pronounced towards the time infected Bam32-/- mice were unable to control parasitemia. The gene discussed is TNF; the disease is parasitic infectious disease.