To further visualize the effect of EOR-Ca2+-ROS-NF-κB on HCV replication, we treated JFH1-infected Huh7.5.1 cells with TMB-8, NAC andSN50 and monitored the intracellular expression of HCV proteins by indirect immunofluorescence at 72 h post-infection. The gene discussed is NFKB1; the disease is infection.