After Newcastle disease virus (NDV) inoculation and R848 injection of pigeons, the expression levels of proinflammatory cytokines and IFN-γ in spleen were significantly up-regulated and TLR7 mRNA significantly increased in the R848-injected group, but significantly decreased in the LaSota-inoculated group at three day post-infection. This evidence concerns the gene TLR7 and infection.