More recently, sera from 24 SN-APS patients were analysed for aPL by TLC immunostaining, for anti-vimentin/cardiolipin antibodies by ELISA, and for anti-annA5 and anti-prothrombin antibodies by ELISA and dot blot, with the aim of identifying the best screening combination to detect aPL in SN-APS patients [50]. This evidence concerns the gene VIM and autoimmune polyendocrinopathy.