The combination of two of the tested methodological approaches, TLC immunostaining for aCL and ELISA for anti-vimentin/cardiolipin complex antibodies, was able to detect aPL/cofactors in about two-thirds of SN-APS patients with thrombosis or pregnancy morbidity, with a small additional gain when also performing ELISA for prothrombin and annA5. Here, F2 is linked to Venous thrombosis.