The expression of FOXO3a and phosphorated-FOXO3a was examined, as well as the subcellular localization of FOXO3a in HCC cells treated with doxorubicin, doxorubicin plus salinomycin or salinomycin alone for 48 h (Fig. 3A and B). The gene discussed is FOXO3; the disease is hepatocellular carcinoma.