These results suggested that doxorubicin treatment significantly upregulated expression of phosphorylated FOXO3a and AKT in HCC cells, and promoted cytoplasm translocalization of FOXO3a, whereas salinomycin reversed the enhanced phosphorylation of FOXO3a and AKT and accelerated nuclear accumulation of FOXO3a (Fig. S4A and B). This evidence concerns the gene AKT1 and hepatocellular carcinoma.