To further investigate the protein expression of AGR2 in post-radiotherapy recurrence HNSCC, chemotherapeutic response and lymph node metastasis of HNSCC, five recurrence cases after radiotherapy and 12 paired HNSCC cases, including presurgical biopsy and postsurgical specimen of two rounds of TPF (cisplatin, docetaxel, and fluorouracil) chemotherapy were selected for immunohistochemistry analysis. Here, AGR2 is linked to head and neck squamous cell carcinoma.