To determine whether over-expression of AGR2 was related to high-grade squamous cell carcinoma in mice, we performed AGR2 immunohistochemistry and found that AGR2 was located mostly in the membrane and cytoplasm of the cancer cell of Tgfbr1 cKO mice and Tgfbr1/Pten 2cKO mice (n = 5, respectively). This evidence concerns the gene AGR2 and squamous cell carcinoma.