Human GBM xenograft tumors were established in athymic nude mice with the human GSC-containing neurospheres N08-30, the human GBM U87MGwtEGFR cell line that overexpresses wtEGFR and the CD133 stem cell marker [42], and the GBM LN229wtEGFR cell line that also overexpresses wtEGFR [43]. The gene discussed is PROM1; the disease is glioblastoma.