Based on the ROC curve, the optimal cutoff value of serum thioredoxin levels as an indicator for auxiliary diagnosis of HCC was projected to be 20.5 ng/mL, which yielded a sensitivity of 84.3% and a specificity of 91.8%, with the area under the curve at 0.946 (95% CI, 0.923–0.969); Table 3 and Figure 2a. Here, TXN is linked to hepatocellular carcinoma.