Using their pharmacological β-cells ablation model, Unger and colleagues generated near complete insulin-deficient mice (in this model insulin level is below 5 pg/mL, which is the threshold of detection of the most sensitive ELISA method used to quantify insulin), and demonstrated that systemic overexpression of leptin alleviates hyperglycemia in these mice (Yu et al., 2008). This evidence concerns the gene INS and Hyperglycemia.