However, we found that AS605240 did not have cytotoxic effects against T-ALL cells at concentrations expected to inhibit solely PI3Kγ (0.1–0.2 μM) [23], being cytotoxic only at concentrations high enough to inhibit more than 70% of p-Akt accumulation (Figure 3b), presumably disrupting the activity of several PI3K isoforms. This evidence concerns the gene AKT1 and acute lymphoblastic leukemia.