Next, to determine whether PAICS or IMPDH2 are essential for PCa cell proliferation, we applied siRNA-mediated knockdown and assessed the proliferation of LNCaP, VCaP and an derivative of LNCaP that has acquired resistance to androgen deprivation, LNCaP-abl [29] using a colourimetric-based assay (MTS). This evidence concerns the gene PAICS and posterior cortical atrophy.