Among genes predicted by three major online miRNA target prediction algorithms, namely TargetScan, PicTar and miRanda, we were particularly interested in disintegrin and metalloproteinase domain-containing protein 9 (ADAM9), HMGA2, RAC1, sal-like 4 (SA LL4), Twist1, CTNNB1, ZEB1, ZEB2, Yes1, which had been reported to regulate tumor EMT, stemness, migration, invasion and tumorigenesis [24–26]. This evidence concerns the gene TWIST1 and neoplasm.