HES6 and cancer: To confirm that HES6 is not under Notch3 control, we used two separate siRNAs to knock down Notch3 expression in 22Rv1 and LnCaP (i.e. the cancer lines with the highest Notch3 and HES6 levels) and measured the protein levels of both HES6 and HES1, a known Notch3 target 34.