TFPI2 and hepatocellular carcinoma: Moreover, changes in gene expression profiles of HCC cells upon treatment with the demethylating agent 5-Aza-2′-deoxycytidine lead Wong and colleagues [23] to identify the Kunitz-type serine protease inhibitor tissue factor pathway inhibitor-2 (TFPI-2) as a new tumor suppressor significantly downregulated in HCCs; TFPI-2 overexpression, indeed, significantly suppressed both proliferation and invasiveness of tumor cells.