TP53 and colorectal carcinoma: Western blotting and immunohistochemistry data did not exhibit statistically significant differences in total p53 protein levels in HCT116FBXW7−/− cells and FBXW7-mutated CRC tissues, compared with parental HCT116 cells which have functional wild-type TP53, and FBXW7-wild-type CRC tissues (Figure 5).