Particularly, these aberrant TF-miRNA networks regulated expression of 18 genes (COL1A1, COL1A2, COL5A2, COL11A1, DSG3, ACHE, SERPINE1, SERPINB2, CXCL5, MMP1, PLAU, SPP1, GJB2, CLDN2, CDKN2A, CENPF, MAD2L1, and NCAM1), most of which (17 out of 18) were up-regulated in gastric cancer tissues (Fig 4). This evidence concerns the gene SPP1 and gastric cancer.