These genes and their informative CpG sites included ZNF300 at CpG6, SLC22A20 at CpG3 and 5 and SHISA7 at CpG1, 5 and 6 (see Figure 6A, B), which could distinguish between HC + CHB and LC + HCC with a lower cut-off value, while distinguishing between HC + CHB + LC and HCC with a higher cut-off value of CpG methylation (Figure 6A). This evidence concerns the gene SHISA7 and hepatocellular carcinoma.