To determine whether the Bcl-2/BAX apoptosis pathway was involved in the enhancement of the 5-FU-related pro-apoptotic effect on HCC cells mediated by PCAF, we examined the impact of 5-FU on the expression of Bcl-2 and BAX in both PLC/PRF/5 PCAF cells and PLC/PRF/5 Vector cells by western immunoblotting. Here, BCL2 is linked to hepatocellular carcinoma.