Actually, the pathway enrichment analysis described above (Fig. 3a) revealed that the functional states of multiple signal transduction and/or transcription pathways, including the Jak-Stat, the Wnt-β catenin, Notch and AKT-mTOR axes, exhibited significant differences when comparing SSEA-4+ to SSEA-4− osteosarcoma cells (Supplementary Fig. 4a,b). This evidence concerns the gene SOAT1 and osteosarcoma.