Given the promising data concerning the AgNPs utilization in anticancer therapy of CNS tumors and the reliable utility of an in ovo model in oncological studies, the aim of this study was to evaluate the impact of AgNPs on proliferation and apoptosis activity of GBM cells cultured in an in ovo model, including the levels of active caspase 9 and active caspase 3. This evidence concerns the gene CASP9 and glioblastoma.