BRD4 and nut midline carcinoma: It is derived from medicinal chemistry optimization of a hit derived from a phenotypic screen to identify small molecules able to enhance ApoA1 expression [72] The fusion between BRD4 (and to a lesser extent BRD3) with NUT gene leads to NMC [73], and this fusion is oncogenic due to the inability to sequester important regulatory molecules such as CBP/p300 into BRD4-NUT nuclear foci, which are formed in a bromodomain-dependent manner, and knockdown of BRD4-NUT with anti-NUT siRNAs has been shown to lead to cell differentiation and apoptosis [74].