To further investigate the effect of ABL inhibition on Notch signalling, real-time PCR was performed to see the effect of IM on Hes1. Results showed that Hes1 was significantly (p<0.01) upregulated in ALL-SIL cells at 48h posttreatment with 10 μM IM (Fig 8b). The gene discussed is HES1; the disease is acute lymphoblastic leukemia.