Consistent with the effects of the Twist1 mutants on E-cadherin repression in NSCLC cells, expression of the R34K mutant also failed to repress E-cadherin in MCF7 cells, as determined by Western blot (Fig. 6C), and indirect immunofluorescence (Fig. 6D). This evidence concerns the gene TWIST1 and non-small cell lung carcinoma.