It was shown earlier that phosphorylation of Twist1 by protein kinase B at Ser42 leads to the inhibition of DNA damage-induced p53 activity (27) and phosphorylation by mitogen-activated protein kinases (MAPKs) at Ser-68, on the contrary, was shown to be important for the stabilization of Twist1 and promotion of breast cancer cell aggressiveness (25). This evidence concerns the gene TWIST1 and breast cancer.