To ensure that the Twist1 arginine methylation-mediated E-cadherin repression was not restricted to the epithelium of the lung, we also tested the effects of wild-type and methylation-deficient mutants of Twist1 on E-cadherin repression in another epithelial cell-derived cancer cell line, MCF7 (a human breast cancer cell line). This evidence concerns the gene CDH1 and breast carcinoma.