To this end, the expression and distribution of GLS1 and GLS2 were determined in a serial set of liver tissues including 20 normal liver (NL), 44 fibrotic liver (FL), 12 focal nodular hyperplasias (FNH), 5 hepatocellular adenoma (HCA), and 10 dysplastic nodules (DN) and compared to expression in 112 HCC samples. Here, GLS2 is linked to hepatocellular carcinoma.