Staining for GLS1 was positive in 94.64% of HCC samples (83/112 were strongly stained and 23/112 were weakly stained), 60% of DN (2/10 were strongly stained and 4/10 were weakly stained), 36.4% of fibrotic liver (1/44 was strongly stained and 15/44 were weakly stained), 20% of HCA (1/5 was weakly stained), 25% of FNH (3/12 were weakly stained), and 10% of normal liver samples (2/20 were weakly stained). This evidence concerns the gene GLS and hepatocellular carcinoma.