ARNT and hepatocellular carcinoma: To confirm whether the changes in cellular morphology were accompanied by the depletion of ARNT, we examined the hepatoma C4 cell line, in which ARNT is degraded by the replacement of Gly326 with asparagine, and vT2 cells, which were derived from C4 cells possessing complete transfection of ARNT cDNA [27] (Figure 1E).