Next, we set out to analyze the HCA mRNA expression in the breast cancer cell lines BT-474 (progesterone receptor (PR) positive, estrogen receptor (ER) positive, HER2/neu positive), HCC1954 (PR negative, ER negative, HER2/neu positive) and HCC38 (PR negative, ER negative, HER2/neu negative) versus the non-tumorigenic epithelial breast cell line MCF12A. This evidence concerns the gene ESR1 and breast carcinoma.