TGFβ1 has been traditionally used to investigate the EMT process and fibrotic response in epithelial cancer cells.2, 27, 28, 29 As shown in Figures 1a and b, p66Shc protein level was significantly decreased by time-dependent TGFβ1 treatment in human bronchial epithelial cells (HBECs) and human lung adenocarcinoma A549 cells, respectively. The gene discussed is TGFB1; the disease is lung adenocarcinoma.