Importantly, the cytolytic capacity of HTNV-Gn/Gc-specific granzyme B-producing CD4+T cells in killing the HTNV-Gn/Gc peptides pulsed autologous B-LCLs was also observed in our experiment, with the stronger cytotoxicity percentage in much milder patients, indicating that HTNV-Gn/Gc-specific effector CD4+T cells against HTNV infection in HFRS patients were comprised of polyfunctional Th1 cells and cytotoxic ThGzmB+ cells. This evidence concerns the gene CD4 and hemorrhagic fever with renal syndrome.