PIM1 and hepatocellular carcinoma: With additional immunohistochemical staining for Carbonic Anhydrase 9 (CA9) and Glucose Transporter 1 (GLUT-1), both being well known HIF targets, in the primary HCC samples, we observed expression of CA9 and/or GLUT-1 in HCC tissues in majority (20/22) of the PIM1-positive cases(Figure 1B).