Using subcutaneous injection of tumor cells in nude mice, knockdown of PIM1 in both HCC cell lines significantly reduced the tumor growth in vivo (Figure 2Aii) in SMMC-7721 and MHCC-97L cell lines, respectively, as compared with their NTC (p=0.003 and 0.036 for SMMC-7721 and MHCC-97L, respectively). This evidence concerns the gene PIM1 and hepatocellular carcinoma.