To test this hypothesis, we investigated the effect of miR-340 on early apoptosis of glioma cells using the Annexin-V/propidium iodide(PI) assay and FCS, and found that the percentage of early apoptosis cells were greatly increased in both A172 and U251 cells transfected with miR-340 (Figure 4A). The gene discussed is ANXA5; the disease is glioma.