TP53 and ductal breast carcinoma in situ: To investigate the pathway-level relationships of the individual mutations, we performed a DAVID analysis (http://david.abcc.ncifcrf.gov) and found that mutated genes in the DCIS-IDC were significantly associated with categories of ‘notch signaling pathway’, ‘cell adhesion’, ‘cell division’, ‘DNA damage response’ and ‘p53 signaling pathway’, while pure DCIS were associated with the ‘mTOR signaling pathway’ and ‘apoptosis’ (Table S7).