Based on our earlier observation that CD133+ cells from a KPC tumor are able to generate tumors at very low cell density [7], in the current study we overexpressed CD133 in MIA PaCa-2 cell line (having all other cancer phenotype, but extremely low CD133 expression) to generate a system in which we can study the downstream effect of CD133 surface expression and how its expression contributes to the cancer stem cell phenotype. The gene discussed is PROM1; the disease is cancer.