In this study, we identified the following: LMO3 is increased in glioma cell lines and tissues and is correlated with a poor prognosis; miR-101 induces apoptosis by suppressing LMO3; and miR-101 inhibits the expression of LMO3 by recovering its methylation status via the regulaton of methylated histone occupation and the suppression of the transcription factors USF and MZF1 in gliomas. Here, USF1 is linked to central nervous system cancer.