Finally, in order to explore the role of LM and LM-induced responses in HCC patients and in normal people, peripheral blood DCs were obtained and infected by LM, then total RNA samples were extracted, and NOD1, NLRP3 and TLR4 expression levels were analyzed by real-time PCR (Fig. 7D). The gene discussed is NLRP3; the disease is hepatocellular carcinoma.