This LM recombinant epitope expresses the fusion peptide MPFG (multiple peptide fusing genes), a combination of HCC-related human leukocyte antigen (HLA)-A0201 epitopes, full-length hepatitis B virus (HBV) core protein (HBc), HBV-X protein (HBx)52-60, HBx140-148, alpha-Fetoprotein (AFP) 158-166 and melanoma antigen gene-A (MAGE)271-279, and has proven to be effective in inducing strong and specific anti-tumor cellular immunity to HCC [16]. The gene discussed is AFP; the disease is hepatocellular carcinoma.