Because CREB1 dysregulation in gastric cancer is still not well understood, we firstly investigated the expression of CREB1 protein by immunohistochemistry (IHC) in a total of 285 paraffin-embedded gastric samples including 185 cases of primary gastric cancer tissues, 50 cases of secondary lymph node metastatic foci and 50 cases of nontumorous gastric mucosa. The gene discussed is CREB1; the disease is gastric cancer.