By using TargetScan, PicTar and miRanda, we predicted putative genes of miR-195 which had not been experimentally identified yet, and finally obtained several putative targets which were reported correlating with tumor growth or metastasis, such as FBXL20, POU2F1, PPPDE2, SCAI, which need further analysis. This evidence concerns the gene POU2F1 and neoplasm.