EGFR and head and neck squamous cell carcinoma: To explore whether the interaction of ANO1 and EGFR was limited to Te11 cells or whether ANO1 and EGFR also interact in other cancer cell lines, we immunoprecipitated EGFR or ANO1 from lysates of OE21 and SCC4 cells, two HNSCC cells lines with amplification and high expression of ANO1, and probed for coimmunoprecipitation of ANO1 and EGFR, respectively, by western blotting (Supplementary Figure 1A).