Taken together, these findings demonstrate that the interaction between ANO1 and EGFR is independent of ANO1′s activity as a CaCC, EGFR-kinase activity or the phosphorylation and dimerization status of EGFR and suggest that ANO1 and EGFR form a constitutive complex in HNSCC cell lines. This evidence concerns the gene ANO1 and head and neck squamous cell carcinoma.