Furthermore, the expression levels of phosphorylated AKT, phosphorylated GSK3β, ZEB-1 and snail-1, all of which are very important for EMT process during cancer progression, were significantly up regulated in Rab3D-MCF-7 cells, but decreased in siRab3D-MDA-MB-231 cells (Fig. 4C). This evidence concerns the gene GSK3B and cancer.