NFKB1 and myocardial ischemia: It has been reported that hearts from TLR4-defctive mice and TLR4 knockout mice display attenuated NF-κB activation and reduced production of pro-inflammatory cytokine (TNF-α, IL-1β and IL-6) following myocardial ischemia with a short period of reperfusion [4], [16], [22].