Furthermore, as UCB effects on HBMEC were also concentration-dependent, we focused on the concentration with the most disruptive potential regarding the integrity of HBMEC (100 μM UCB in the presence of 100 μM human serum albumin), which corresponds to the bilirubin:albumin ratio that induces acute bilirubin encephalopathy and kernicterus (Brito et al., 2012; Iskander et al., 2014) and to free bilirubin values found in jaundiced neonates (Brito, 2001; Ahlfors et al., 2009). The gene discussed is ALB; the disease is Encephalopathy.